Allowing a season-high eight runs for the loss, Colon lasted only two-thirds of an inning, the shortest start of his career.
He'll get back on the mound Tuesday against the Rays at Tropicana Field.
Watching him warm up and throw a side session since the outing, pitching coach Larry Rothschild said Colon hasn't favored the hamstring and his velocity hasn't diminished.
"As far as throwing the ball, I don't think it's affected him," Rothschild said. "I think it's more when he goes to cover first, in that. In between now, he's kind of tested it a little bit, and I think he's pretty confident in what he can do with it. Hopefully that's just a thing of the past."
Manager Joe Girardi also sees the start as a crucial step in getting Colon back on track after two straight losses.
"I think it's important for him, because his last two nights, the starts have not been his best, and as I said, the start in Toronto, we didn't help him," Girardi said. "We created a lot of that mess for him. Who knows what he would've done, but I still think it's important."
Colon faces a well-rested Jeremy Hellickson, who will make his first start in 16 days.
Hellickson (8-7, 3.21 ERA) was originally scheduled to pitch against the Yankees on July 8, but the game was rained out. This time around will mark the right-hander's first career start against New York.
"It has been a long time. I'm ready and excited to get back out there," Hellickson said. "It has kind of gone fast. But it has been a while."
His last time out against the Cardinals on July 3, Hellickson tossed 7 1/3 innings, giving up three runs on six hits for the win.
"I felt good in the 'pen. The last few times I went this long [without pitching], the command was shaky to begin with," Hellickson said. "Once I got into a rhythm, it kind of came to me. I just got to get into a rhythm early."
Yankees: Pena lands on DL
Ramiro Pena woke up Monday morning -- his 26th birthday -- with severe stomach pains and was taken to the hospital where he was diagnosed with an appendicitis. Because doctors removed his appendix, Pena was forced to go on the disabled list Monday. To fill his spot on the active roster, the Yankees called up Brandon Laird from Triple-A Scranton/Wilkes-Barre. "It'll be a birthday I'm sure he'll never forget," Yankees manager Joe Girardi said of Pena.
After participating in baseball activities for a few weeks at the Yankees' Minor League complex, Eric Chavez was cleared to play his first rehab game on Tuesday for Class-A.
Rays: Torres recalled for bullpen help
With eight relievers seeing action in Sunday's 16-inning loss to the Red Sox, the Rays made a move to add bullpen depth. Making room for Monday's starter Alex Cobb, the club also recalled left-hander Alex Torres. To make space for the pair, the Rays placed Juan Cruz on the 15-day disabled list with a right groin strain and designated Adam Russell for assignment.
Rays pitcher Wade Davis, who is on the 15-day disabled list with a strained right forearm, threw another bullpen session of about 50 to 60 pitches before Monday's game. He hopes to return to the mound for Tampa Bay on Saturday against the Royals, but Joe Maddon said that was unlikely.
After a slew of moves to the bullpen Monday, the Rays have four left-handers in the bullpen for the first time since the end of the 2004 season with expanded rosters.
In Sunday's 1-0 loss to the Red Sox, the Rays went a combined 3-for-50, the first Major League team to record 50 or more at-bats and three or fewer hits in a game going back to 1919.
The Yankees are a Major League-best 28-5 (.848) in day games in 2011.